ANO2 Polyclonal Antibody

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IHC image of ANO2 Polyclonal Antibody diluted at 1:600 and staining in paraffin-embedded human small intestine tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with ANO2 Polyclonal Antibody at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Application:	ELISA, IF, IHC, WB
Areas of Research:	Signal Transduction
Clonality:	Polyclonal
Conjugate:	Unconjugated
Host:	Rabbit
Isotype:	IgG
Purification:	Protein G Purified
Reactivity:	Human, Rat
Trial Size Available:	Yes
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A69283-020	20 µg	$99.00
A69283-050	50 µg	$249.00
A69283-100	100 µg	$399.00

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Product Overview
User Guide & MSDS

Product Overview

Background
Calcium-activated chloride channel (CaCC) which may play a role in olfactory signal transduction. Odorant molecules bind to odor-sensing receptors (OSRs), leading to an increase in calcium entry that activates CaCC current which amplifies the depolarization of the OSR cells, ANO2 seems to be the underlying chloride channel involved in this process. May mediate light perception amplification in retina.

Description
ANO2 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human, Rat

Isotype
IgG

Uniprot ID
Q9NQ90

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Anoctamin-2 protein (1-83AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Anoctamin-2, Transmembrane protein 16B, ANO2, C12orf3, TMEM16B

Application
ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

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ANO2 Polyclonal Antibody