DescriptionMouse monoclonal antibody raised against a partial recombinant ACTL6A, clone 3C4.
Size100 µg
PurificationProtein A purified
ImmunogenACTL6A (NP_817126, 1 a.a. ~ 81 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross ReactivityHuman
IsotypeIgG1 Kappa
FormulationIn 1x PBS, pH 7.2
StorageAliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.
Handling RecommendationsFor maximum recovery of the products, centrifuge the vial prior to opening the cap.
Alternative Names53 kDa BRG1 associated factor A antibody, Actin like 6A antibody, Actin related protein Baf53a antibody, ACTL 6 antibody, Arp4 antibody, ARPN BETA antibody, ArpNbeta antibody, BAF53 antibody, BRG1 associated factor antibody, hArpN beta antibody, INO80K antibody, MGC5382 antibody
Applications & Suggested Dilutions*Western Blot: 0.5-1 µg/mlELISA: 0.25-1 µg/ml
*The end user is responsible for determining optimal working dilutions.
Quality Control Western Blot detection against Immunogen (34.91 KDa).
Note: This item was formerly catalog no. ABB-2026 (ABB2026)
Applications
Western Blot
Western Blot analysis of ACTL6A expression in transfected 293T cell line by ACTL6A Monoclonal Antibody, clone 3C4.Lane 1: ACTL6A transfected lysate (47.5 KDa).Lane 2: Non-transfected lysate.
ELISA
Detection limit for recombinant GST tagged ACTL6A is approximately 0.3 ng/ml as a capture antibody.
Immunohistochemistry (IHC)
Immunofluorescence