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ACTA1 Recombinant Monoclonal Antibody [25E3]

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Overlay histogram showing Hela cells stained with ACTA1 rMAb (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0. 3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
Immunofluorescence staining of HepG2 cells with ACTA1 rMAb at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0. 2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
IHC image of ACTA1 rMAb diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, Raw264. 7 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat brain tissue, Rat heart tissue
All lanes: Actin antibody at 0. 95ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Application: ELISA, FC, IF, IHC, WB
Areas of Research: Cellular Structure
Clonality: Monoclonal
Conjugate: Unconjugated
Host: HEK293F Cell
Isotype: Rabbit IgG
Purification: Affinity Chromatography
Reactivity: Human
Recombinant: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A74129-05050µl $299.00 
A74129-100100µl $399.00 
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Product Overview

Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Description
ACTA1 Recombinant Monoclonal Antibody [25E3]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
Rabbit IgG

Uniprot ID
P68133

Purification
Affinity Chromatography

Immunogen
A synthesized peptide

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA

Application
ELISA, WB, IHC, IF, FC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500, IF:1:30-1:200

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

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