DescriptionMouse monoclonal antibody raised against a partial recombinant TP53, clone 2C11.
Size100 µg
PurificationProtein A purified
ImmunogenTP53 (AAH03596, 94 a.a. ~ 202 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross ReactivityHuman
IsotypeIgG1 Kappa
FormulationIn 1x PBS, pH 7.2
StorageAliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.
Handling RecommendationsFor maximum recovery of the products, centrifuge the vial prior to opening the cap.
Alternative NamesAntigen NY-CO-13, BCC7, Cellular tumor antigen p53, Cys 51 Stop, FLJ92943, HGNC11998, LFS1, Mutant tumor protein 53, p53, p53 cellular tumor antigen, p53 tumor suppressor, P53_HUMAN, Phosphoprotein p53, Transformation related protein 53, TRP53, Tumor protein p53
Applications & Suggested Dilutions*Western Blot: 0.5-1 µg/mlELISA: 0.25-1 µg/mlImmunohistochemistry (IHC): 2.5 µg/ml
*The end user is responsible for determining optimal working dilutions.
Quality ControlWestern Blot detection against Immunogen (37.99 KDa).
Note: This item was formerly catalog no. ABB-6087 (ABB6087)
Applications
Western Blot
TP53 Monoclonal Antibody, clone 2C11. Western Blot analysis of TP53 expression in A-431.
Western Blot analysis of TP53 expression in transfected 293T cell line by TP53 Monoclonal Antibody, clone 2C11.Lane 1: TP53 transfected lysate (43.34 KDa).Lane 2: Non-transfected lysate.
ELISA
Immunohistochemistry (IHC)
Immunofluorescence