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SETD3 Polyclonal Antibody

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IHC image of SETD3 Polyclonal Antibody diluted at 1:400 and staining in paraffin-embedded human appendix tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of SETD3 Polyclonal Antibody diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Application: ELISA, IHC
Areas of Research: Histone Methylation & Demethylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A69963-02020 µg $99.00 
A69963-05050 µg $249.00 
A69963-100100 µg $399.00 
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Product Overview

Background
Histone methyltransferase that methylates 'Lys-4' and 'Lys-36' of histone H3 (H3K4me and H3K36me). Acts as a transcriptional activator. Plays an important role in the transcriptional regulation of muscle cell differentiation via interaction with MYOD1.

Description
SETD3 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
Q86TU7

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Histone-lysine N-methyltransferase setd3 protein (166-265AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Histone-lysine N-methyltransferase setd3, SET domain-containing protein 3, SETD3, C14orf154

Application
ELISA, IHC; Recommended dilution: IHC: 1:200-1:500

User Guide & MSDS

[User Guide]*
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