BackgroundSAP30 (Sin3A-associated protein, 30kDa) is a member of the histone deacetylase complex which consists of SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. It is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones, since histone-binding proteins RBBP7 and RBBP4 are unable to gain access to nucleosomal histones.
DescriptionMouse monoclonal antibody raised against a partial recombinant SAP30, clone 4D2.
Size100 µg
PurificationProtein A purified
ImmunogenSAP30 (NP_003855, 131 a.a. ~ 220 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross ReactivityHuman
IsotypeIgG2b Kappa
FormulationIn 1x PBS, pH 7.2
StorageAliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.
Handling RecommendationsFor maximum recovery of the products, centrifuge the vial prior to opening the cap.
Alternative Names30 kDa Sin3-associated polypeptide antibody, Histone deacetylase complex subunit SAP 30 antibody, MSin3A associated polypeptide p30 antibody, Sin3 corepressor complex subunit SAP 30 antibody, Sin3A associated protein 30kDa antibody
Applications & Suggested Dilutions*Western Blot: 0.5-1 µg/mlELISA: 0.25-1 µg/mlImmunohistochemistry (IHC): 2.5 µg/ml
*The end user is responsible for determining optimal working dilutions.
Quality ControlWestern Blot detection against Immunogen (35.9 KDa).
Note: This item was formerly catalog no. ABB-4063 (ABB4063)
Applications
Western Blot
Applicable
ELISA
Immunohistochemistry (IHC)