RPS6KA2 Monoclonal Antibody [1F6]

Description
Mouse monoclonal antibody raised against a partial recombinant RPS6KA2, clone 1F6.

Size
100 µg

Purification
Protein A purified

Immunogen
RPS6KA2 (AAH02363, 631 a.a. ~ 733 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG2a Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Alternative Names
90 kDa ribosomal protein S6 kinase 2 antibody, HU 2 antibody, KS6A2_HUMAN antibody, MAP kinase activated protein kinase 1c antibody, MAPK-activated protein kinase 1c antibody, MAPKAP kinase 1c antibody, MAPKAPK1C antibody, Mitogen.activated protein kinase-activated protein kinase 1C antibody, p90 RSK3 antibody, p90RSK 2 antibody, pp90RSK 3 antibody, pp90RSK3 antibody, Ribosomal protein S6 kinase alpha-2 antibody, ribosomal protein S6 kinase 90kDa polypeptide 2 antibody, Ribosomal S6 kinase 3 antibody, RSK 3 antibody, RSK antibody, S6K alpha 2 antibody, S6K alpha antibody

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunohistochemistry (IHC): 2.5 µg/ml
Immunofluorescence: 5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (37.33 KDa).

Note: This item was formerly catalog no. ABB-6058 (ABB6058)

Applications

Western Blot	RPS6KA2 Monoclonal Antibody, clone 1F6. Western Blot analysis of RPS6KA2 expression in HeLa NE. Western Blot analysis of RPS6KA2 expression in transfected 293T cell line by RPS6KA2 Monoclonal Antibody, clone 1F6. Lane 1: RPS6KA2 transfected lysate (83.2 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation	Not determined
ELISA	Applicable
Immunohistochemistry (IHC)	Immunoperoxidase of monoclonal antibody to RPS6KA2 on formalin-fixed paraffin-embedded human cerebral cortex. [Antibody Concentration: 3 µg/ml]
Immunofluorescence	Immunofluorescence of monoclonal antibody to RPS6KA2 on HeLa cell. [Antibody Concentration: 10 µg/ml]