DescriptionMouse monoclonal antibody raised against a full length recombinant RAD51, clone 2E5-E5.
Size100 µg
PurificationProtein A purified
ImmunogenRAD51 (AAH01459, 1 a.a. ~ 243 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross ReactivityHuman
IsotypeIgG1 Kappa
FormulationIn 1x PBS, pH 7.2
StorageAliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.
Handling RecommendationsFor maximum recovery of the products, centrifuge the vial prior to opening the cap.
Alternative NamesBRCA1/BRCA2 containing complex subunit 5, BRCC5, DNA repair protein RAD51 homolog 1, E coli RecA homolog, HGNC:9817, Homolog of E coli RecA, homolog of S cerevisiae RAD51, HRAD51, HsRad51, HsT16930, MRMV2, RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51 homolog A, RAD51 homolog S. cerevisiae, RAD51 recombinase, RAD51_HUMAN, RAD51A, RECA, RecA homolog E. coli, RecA like protein, recombination protein A
Applications & Suggested Dilutions*Western Blot: 0.5-1 µg/mlELISA: 0.25-1 µg/ml
*The end user is responsible for determining optimal working dilutions.
Quality ControlWestern Blot detection against Immunogen (52.73 KDa).
Note: This item was formerly catalog no. ABB-6045 (ABB6045)
Applications
Western Blot
RAD51 Monoclonal Antibody, clone 2E5-E5. Western Blot analysis of RAD51 expression in HeLa.
Western Blot analysis of RAD51 expression in transfected 293T cell line by RAD51 Monoclonal Antibody, clone 2E5-E5.Lane 1: RAD51 transfected lysate (37 KDa).Lane 2: Non-transfected lysate.
ELISA
Immunohistochemistry (IHC)
Immunofluorescence