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PARP1 Polyclonal Antibody

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Figure: Western blot using rabbit polyclonal anti PARP1. Lane 1: recombinant human PARP1. Lane 2: Total HeLa cell extract. Lane 3: Total MEF PARP1+/+ cell extract. Lane 4: Total MEF PARP1-/- cell extract. Lane 5: Lysate (50 μg) from HeLa cells. Lane 6: Lysate (50 μg) from HeLa PARP-1sh cells. Lane 7: Lysate (50 μg) from HeLa cells treated for 8 hours with Doxorubicin 5 μg/ml. Lane 8: Lysate (50 μg) from HEK293 cells. Lane 9: Lysate (50 μg) from HEK293 cells. Lane 10: Lysate (50 μg) from HEK293 cells transfected with PARP-1 siRNA. Lane 11: Lysate (50 μg) from HEK293 cells transfected with control siRNA.
Application: ELISA, ICC, IHC, IP, WB
Areas of Research: DNA Damage & Repair
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Purification: Serum
Reactivity: Bovine, Does not cross-react with PARP2, Human, Monkey, Mouse
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-6409-100100 µl $421.00  Discontinued
Availability: Discontinued 
Product Overview

Description
Rabbit polyclonal antibody to PARP1

Formulation
Liquid. Neat serum containing 0.1% sodium azide.

Immunogen
Recombinant human PARP-1 (poly(ADP-ribose) polymerase-1) (aa 1-1014).

Specificity
Human, Mouse, Bovine, Monkey, Does not cross-react with PARP2

Purification
Serum

Storage
Store at +4°C. Avoid multiple freeze/thaw cycles.

Alternative Names
Poly(ADP-ribose) polymerase-1

Application
ELISA, ICC (1:4000), IHC, IP (1:400), WB (1:4000)

Fig. 1.  Figure: Western blot using rabbit polyclonal anti PARP1. Lane 1: recombinant human PARP1. Lane 2: Total HeLa cell extract. Lane 3: Total MEF PARP1+/+ cell extract. Lane 4: Total MEF PARP1-/- cell extract. Lane 5: Lysate (50 μg) from HeLa cells. Lane 6: Lysate (50 μg) from HeLa PARP-1sh cells. Lane 7: Lysate (50 μg) from HeLa cells treated for 8 hours with Doxorubicin 5 μg/ml. Lane 8: Lysate (50 μg) from HEK293 cells. Lane 9: Lysate (50 μg) from HEK293 cells. Lane 10: Lysate (50 μg) from HEK293 cells transfected with PARP-1 siRNA. Lane 11: Lysate (50 μg) from HEK293 cells transfected with control siRNA.

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