Rabbit polyclonal antibody to PARP1
Liquid. Neat serum containing 0.1% sodium azide.
Recombinant human PARP-1 (poly(ADP-ribose) polymerase-1) (aa 1-1014).
Human, Mouse, Bovine, Monkey, Does not cross-react with PARP2
Store at +4°C. Avoid multiple freeze/thaw cycles.
ELISA, ICC (1:4000), IHC, IP (1:400), WB (1:4000)
Fig. 1. Figure: Western blot using rabbit polyclonal anti PARP1. Lane 1: recombinant human PARP1. Lane 2: Total HeLa cell extract. Lane 3: Total MEF PARP1+/+ cell extract. Lane 4: Total MEF PARP1-/- cell extract. Lane 5: Lysate (50 μg) from HeLa cells. Lane 6: Lysate (50 μg) from HeLa PARP-1sh cells. Lane 7: Lysate (50 μg) from HeLa cells treated for 8 hours with Doxorubicin 5 μg/ml. Lane 8: Lysate (50 μg) from HEK293 cells. Lane 9: Lysate (50 μg) from HEK293 cells. Lane 10: Lysate (50 μg) from HEK293 cells transfected with PARP-1 siRNA. Lane 11: Lysate (50 μg) from HEK293 cells transfected with control siRNA.