HDAC8 Monoclonal Antibody [2F4]

Background
Histone deacetylases (HDACs) play a critical role in transcriptional repression of gene expression in eukaryotic cells through catalyzing the hydrolytic removal of acetyl groups from histone lysine residues. Three distinct families of HDACs have been described, comprising a group of at least 20 proteins in humans. HDAC8 is a class I histone deacetylase.

Description
Mouse monoclonal antibody raised against a full length recombinant HDAC8, clone 2F4.

Purification
Protein A Purified

Size
100 µg

Immunogen
HDAC8 (AAH50433, 1 a.a. ~ 378 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG2b Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Alternative Names
CDA07 antibody, HD8 antibody, HDACL 1 antibody, Histone deacetylase 8 antibody, Histone deacetylase like 1 antibody, RPD 3 antibody

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunofluorescence: 5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (67.58 KDa).

Note: This item was formerly catalog no. ABB-4008 (ABB4008)

Applications

Western Blot	HDAC8 Monoclonal Antibody [2F4]. Western Blot analysis of HDAC8 expression in Hela NE. Western Blot analysis of HDAC8 expression in transfected 293T cell line by HDAC8 Monoclonal Antibody [2F4]. Lane 1: HDAC8 transfected lysate (41.8 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation	Not determined
ELISA	Detection limit for recombinant GST tagged HDAC8 is approximately 0.3 ng/ml as a capture antibody.
Immunohistochemistry (IHC)	Not determined
Immunofluorescence	Immunofluorescence of monoclonal antibody to HDAC8 on HeLa cell.  [Antibody Concentration: 10 µg/ml]