BackgroundHistone deacetylases (HDACs) play a critical role in transcriptional repression of gene expression in eukaryotic cells through catalyzing the hydrolytic removal of acetyl groups from histone lysine residues. Three distinct families of HDACs have been described, comprising a group of at least 20 proteins in humans. HDAC11 is a novel class I histone deacetylase.
DescriptionMouse monoclonal antibody raised against a full length recombinant HDAC11, clone 1C2-2G5.
Size100 µg
PurificationProtein A purified
ImmunogenHDAC11 (AAH09676, 1 a.a. ~ 348 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross ReactivityHuman
IsotypeIgG1 Kappa
FormulationIn 1x PBS, pH 7.2
StorageAliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.
Handling RecommendationsFor maximum recovery of the products, centrifuge the vial prior to opening the cap.
Alternative NamesFLJ22237 antibody, HD 11 antibody, Histone deacetylase 11 antibody
Applications & Suggested Dilutions*Western Blot: 0.5-1 µg/mlELISA: 0.25-1 µg/mlImmunofluorescence: 5 µg/ml
*The end user is responsible for determining optimal working dilutions.
Quality ControlWestern Blot detection against Immunogen (64.28 KDa).
Note: This item was formerly catalog no. ABB-4011 (ABB4011)
Applications
Western Blot
HDAC11 Monoclonal Antibody [1C2-2G5]. Western Blot analysis of HDAC11 expression in HL-60.
Western Blot analysis of HDAC11 expression in transfected 293T cell line by HDAC11 Monoclonal Antibody [1C2-2G5].Lane 1: HDAC11 transfected lysate (39.2 KDa).Lane 2: Non-transfected lysate.
ELISA
Immunohistochemistry (IHC)