Granzyme B Monoclonal Antibody [B18.1], FITC Conjugated

Background
Granzymes B is serine proteases family member that is found in the granules of cytotoxic T cells (CTLs) and natural killer (NK) cells. Once a target cell is recognized, CTLs and NK cells secrete GrB and other granule proteins, including perforin, into the immunological synapse. GrB cleaves and activates, or inactivates, various protein substrates, resulting in the eventual apoptotic demise of the target cell. GrB apoptosis is also activated by adenovirus, which can effectively replace perforin. Extracellular granzyme B has been associated in the pathogenesis of vascular leakage, atherosclerosis aneurysm, chronic wound healing, and skin aging.

Formulation
Liquid. In PBS containing 0.02% sodium azide.

Immunogen
Recombinant human granzyme B (aa 7-227).

Specificity
Human

Isotype
IgG1

Purification
Serum

Storage
Store at 4°C. Avoid multiple freeze/thaw cycles.

Application
1µl of a 1mg/ml stock solution were used to stain 200'000 cells in a sample volume of 50µl, FC, ICC, IHC

Fig. 1. Detection of granzyme B in permeabilized human CD8+ T lymphocytes (filled profile). Isotype control in combination with a secondary FITC-labeled antibody is shown as control.