Epigenetic Research Papers 2/16/25 - 2/21/25

Yang C et. al. (February 2025). The Diminution of R-Loops Generated by LncRNA DSP-AS1 Inhibits DSP Gene Transcription to Impede the Re-Epithelialization During Diabetic Wound Healing Adv Sci (Weinh). :e2406021.
This study investigates how the downregulation of lncRNA DSP-AS1 impairs re-epithelialization in diabetic wound healing by reducing R-loop formation and inhibiting DSP gene transcription. DSP-AS1 normally facilitates TET3-mediated DNA demethylation at the DSP promoter, promoting DSP expression, which is essential for wound closure. The findings suggest that restoring DSP-AS1 activity could be a potential therapeutic strategy for improving diabetic wound healing.
Products Used: Methylamp Methylated DNA Capture (MeDIP) Kit, EpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit

Bai H et. al. (February 2025). MyD88 inhibitor TJ-M2010-5 alleviates spleen impairment and inflammation by inhibiting the PI3K/miR-136-5p/AKT3 pathway in the early infection of Trichinella spiralis Vet Res. 56(1):28.
This study examines the effects of the MyD88 inhibitor TJ-M2010-5 in alleviating spleen impairment and inflammation during early Trichinella spiralis infection. The findings show that TJ-M2010-5 reduces inflammation by inhibiting the PI3K/miR-136-5p/AKT3 pathway, promoting a shift in macrophage polarization, and improving histological damage. These results suggest that TJ-M2010-5 could be a potential therapeutic strategy for managing early-stage T. spiralis infections.
Products Used: Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric), EpiQuik DNMT Activity/Inhibition ELISA Easy Kit (Colorimetric)

Chen H et. al. (February 2025). LsBLH2-LsOFP6-LsKANT3 module regulates bolting by orchestrating the gibberellin biosynthesis and metabolism in lettuce Plant Biotechnol J.
This study explores the genetic regulation of bolting time in lettuce, focusing on the LsBLH2 gene, which controls gibberellin biosynthesis and metabolism. The research reveals that LsBLH2, in interaction with LsOFP6 and LsKNAT3, regulates bolting by modulating the accumulation of bioactive gibberellins during the vegetative-to-reproductive phase transition. These findings provide new insights into bolting regulation and potential strategies for breeding lettuce varieties that resist premature bolting.
Products Used: EpiQuik Plant ChIP Kit

Li X et. al. (February 2025). CRF12 specifically regulates the flowering time of Arabidopsis thaliana under non-inductive conditions Plant J. 121(3):e17257.
This study investigates the role of CYTOKININ RESPONSE FACTORS 12 (CRF12) in regulating flowering time in Arabidopsis thaliana under non-inductive conditions. The research reveals that CRF12 interacts with CONSTANS (CO) and TARGET OF EAT 1/2 (TOE1/2) to regulate flowering under a 12-hour light/dark cycle and inhibits TOE1/2 activity under short-day conditions, providing new insights into the flowering regulatory network in non-inductive environments.
Products Used: EpiQuik Plant ChIP Kit

Zhang H et. al. (February 2025). Evolutionary-Distinct Viral Proteins Subvert Rice Broad-Spectrum Antiviral Immunity Mediated by the RAV15-MYC2 Module Adv Sci (Weinh). :e2412835.
This study explores how evolutionarily distinct viral proteins disrupt the rice broad-spectrum antiviral immunity mediated by the RAV15-MYC2 module. The research reveals that viral proteins from different rice viruses target the OsRAV15 transcription factor and interfere with its interaction with the OsMYC2-OsJAZ complex, thereby suppressing jasmonic acid (JA)-mediated immunity and facilitating viral infection.
Products Used: EpiQuik Plant ChIP Kit

Wen YL et. al. (March 2025). Transcriptomic Regulation by Astrocytic m6A Methylation in the mPFC Wen YL et. al. (March 2025).
This study investigates the role of m6A methylation in astrocyte-mediated gene expression in the medial prefrontal cortex (mPFC). It demonstrates that ALKBH5, an m6A demethylase, regulates transcription and gene expression in astrocytes, influencing brain function. These findings suggest the importance of m6A modification in astrocytes and its potential implications for brain disorders.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Zhang S et. al. (February 2025). Oncometabolite D-2HG drives tumor metastasis and protumoral macrophage polarization by targeting FTO/m6A/ANGPTL4/integrin axis in triple-negative breast cancer Zhang S et. al. (February 2025).
This study examines the role of the oncometabolite D-2HG in promoting tumor metastasis and macrophage polarization in triple-negative breast cancer (TNBC). It reveals that D-2HG enhances m6A RNA modifications, specifically on ANGPTL4 mRNA, by inhibiting FTO, which boosts ANGPTL4 translation and activates integrin-mediated signaling to promote tumor growth. Additionally, ANGPTL4 contributes to M2 macrophage polarization, further advancing TNBC progression, highlighting potential therapeutic targets for TNBC treatment.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)