Epigenetic Research Papers 12/23/24 - 12/27/24

Casarotto LT et. al. (December 2024). Late-gestation heat stress alters placental structure and function in multiparous dairy cows J Dairy Sci.
This study investigates the effects of late-gestation heat stress (HT) on placental structure and function in multiparous dairy cows. HT reduced gestation length, calf birth weight, placental weight, and cotyledon number, while increasing placental efficiency and vascular area. HT altered 289 placental genes, affecting pathways related to nutrient transport, gas exchange, and inflammatory responses, and caused hypermethylation in genes regulating tissue organization. These findings highlight HT’s negative impacts on fetal development and dam lactation, emphasizing the importance of managing gestational heat stress to improve dairy cow resilience and productivity.
Products Used: Methylamp DNA Modification Kit

Zhang Y et. al. (December 2024).  AEBP1 Silencing Protects Against Cerebral Ischemia/Reperfusion Injury by Regulating Neuron Ferroptosis and Microglia M2 Polarization Through PRKCA-PI3K-Akt Axis Drug Dev Res. 85(8):e70032.
This study examines the role of AEBP1 silencing in protecting against cerebral ischemia/reperfusion injury. AEBP1 downregulation mitigates neuron ferroptosis by increasing cell viability and antioxidant defenses while reducing oxidative stress and iron accumulation. It also promotes anti-inflammatory microglia M2 polarization by enhancing CD206 expression and reducing pro-inflammatory cytokines. Mechanistically, AEBP1 silencing restores PRKCA expression, which activates the PI3K/Akt pathway, further reducing neuronal damage and infarct size in vivo. These findings highlight AEBP1 as a potential therapeutic target for alleviating cerebral ischemia/reperfusion injury.
Products Used: EpiQuik Chromatin Immunoprecipitation (ChIP) Kit

Marques O et. al. (December 2024). Inflammation-driven NFκB signaling represses Ferroportin transcription in macrophages via HDAC 1 and 3 Blood.
This study explores how inflammation-driven NFκB signaling represses Ferroportin (FPN) transcription in macrophages. It identifies HDAC1 and HDAC3 as key mediators recruited to the FPN promoter's antioxidant response element (ARE), where they suppress transcription downstream of NFκB. These findings reveal a critical mechanism linking inflammation to iron dysregulation and provide potential therapeutic targets for anemia of inflammation.
Products Used: EpiNext CUT&RUN Fast Kit

Jia X et. al. (December 2024). METTL16 controls airway inflammations in smoking-induced COPD via regulating glutamine metabolism Ecotoxicol Environ Saf. 289:117518.
This study investigates the role of METTL16 in regulating airway inflammation in smoking-induced COPD. The findings reveal that decreased METTL16 levels exacerbate inflammation by destabilizing GOT2 via m6A modification, disrupting glutamine metabolism in lung epithelial cells. Supplementing glutamine reduced inflammation in a COPD murine model, highlighting the therapeutic potential of targeting the METTL16/GOT2/glutamine axis in COPD management.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Tao M et. al. (December 2024). METTL3-mediated m6A modification of EGR1 mRNA promotes T2DM vasculopathy Tao M et. al. (December 2024).
This study examines how METTL3-mediated m6A modification contributes to vascular lesions in Type 2 diabetes mellitus (T2DM). The findings reveal that METTL3 promotes endothelial-to-mesenchymal transition (EndMT), proliferation, and migration in endothelial cells by targeting EGR1 mRNA. Targeting METTL3 offers a potential therapeutic strategy for managing diabetic vascular complications.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Zhang Q et. al. (December 2024). YTHDF2 promotes arsenic-induced malignant phenotypes by degrading PIDD1 mRNA in human keratinocytes Zhang Q et. al. (December 2024). 
This study explores the role of YTHDF2 in arsenic-induced carcinogenesis in human keratinocytes. The findings reveal that YTHDF2 degrades PIDD1 mRNA via m6A modification, suppressing caspase-2-mediated apoptosis and promoting malignant phenotypes in arsenic-exposed cells. These results shed light on the molecular mechanisms underlying arsenic-induced skin cancer.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Jia X et. al. (December 2024). METTL16 controls airway inflammations in smoking-induced COPD via regulating glutamine metabolism Ecotoxicol Environ Saf. 289:117518.
This study investigates the role of METTL16 in smoking-induced COPD and its regulation of glutamine metabolism. The findings show that METTL16 depletion exacerbates airway inflammation by destabilizing GOT2 mRNA and altering glutamine metabolism, suggesting that targeting METTL16 could offer therapeutic potential for treating COPD-related inflammation.
Products Used: EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit