Epigenetic Research Papers 10/30/23 - 11/3/23

Zhang L et. al. (October 2023). Acetyltransferases CBP/p300 control transcriptional switch of β-catenin and Stat1 promoting osteoblast differentiation J Bone Miner Res.
The article explores the role of histone acetyltransferases CBP and p300 in bone homeostasis. Conditional knockout of CBP or p300 in osteoblasts reduces bone mass and strength, mainly by suppressing bone formation. Their acetyltransferase activity is crucial for promoting osteogenesis by activating β-catenin and inhibiting Stat1. Epigenetic mechanisms involving histone H3K27 acetylation and Foxo1 are identified. This study uncovers critical regulators of osteoblast differentiation and bone homeostasis.
Products Used: EpiQuik Total Histone Extraction Kit

Song N et. al. (October 2023).  Synergistic induction of phytoalexins in Nicotiana attenuata by JA and ethylene signaling mediated by NaWRKY70 J Exp Bot.
The study investigates the coordinated regulation of phytoalexins in Nicotiana attenuata when exposed to both jasmonate (JA) and ethylene signaling, in response to the pathogen Alternaria alternata. Researchers identify the key role of the transcription factor NaWRKY70 in synergistically inducing phytoalexins. Their findings provide insights into the plant's defense mechanisms against A. alternata, highlighting the interplay of JA and ethylene signaling mediated by NaWRKY70.
Products Used: EpiQuik Plant ChIP Kit

Wang T et. al. (October 2023). The transcription factor MYB110 regulates plant height, lodging resistance, and grain yield in rice Plant Cell.
This study reveals that the transcription factor MYB110 plays a crucial role in regulating plant height, lodging resistance, and grain yield in rice. MYB110 is influenced by phosphate (Pi) availability, acting as a negative regulator of plant height. Inactivating MYB110 enhances lodging resistance and boosts grain yield, making it a potential target for increasing crop productivity.
Products Used: EpiQuik Plant ChIP Kit

Zhang QC et. al. (October 2023). Phenethyl isothiocyanate inhibits metastasis potential of non-small cell lung cancer cells through FTO mediated TLE1 m6A modification Acta Pharmacol Sin.
The study demonstrates how phenethyl isothiocyanate (PEITC) hinders lung cancer cell metastasis. PEITC reduces cell viability, migration, and invasion in non-small cell lung cancer (NSCLC) cells by increasing m6A methylation and inhibiting FTO, an m6A demethylase. FTO influences TLE1, a transcriptional co-repressor, impacting its stability and expression. This research provides insights into a novel strategy to inhibit lung cancer cell metastasis by targeting m6A methylation.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Peng Y et. al. (October 2023). N6-methyladenosine reader protein IGF2BP1 suppresses CD8 + T cells-mediated tumor cytotoxicity and apoptosis in colon cancer Apoptosis.
This study uncovers the role of the m6A reader protein IGF2BP1 in colon cancer's immune escape. IGF2BP1 is linked to poor clinical outcomes in colon cancer patients. It inhibits CD8+ T cell-mediated antitumor immunity by stabilizing PD-L1 mRNA. PD-L1, a target of IGF2BP1, reduces CD8+ T cell tumor cytotoxicity, promoting immune escape. These findings suggest the potential of m6A-targeted immune checkpoint blockade in colon cancer therapy, offering new insights for precision treatment.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Chen X et. al. (October 2023). Targeting YTHDF2 inhibits tumorigenesis of diffuse large B-cell lymphoma through ACER2-mediated ceramide catabolism J Adv Res.
This article investigates YTHDF2, an epigenetic regulator, in diffuse large B-cell lymphoma (DLBCL). YTHDF2 is highly expressed in DLBCL and correlates with poor prognosis. YTHDF2 overexpression promotes DLBCL progression, while YTHDF2 knockout inhibits tumor growth. YTHDF2 regulates ceramide metabolism through ACER2, impacting the balance of ceramides and sphingosine-1-phosphate (S1P), ultimately driving DLBCL tumorigenesis. These findings suggest YTHDF2 as a potential therapeutic target in DLBCL.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Guo X et. al. (October 2023). Hypoxia-induced neuronal activity in glioma patients polarizes microglia by potentiating RNA m6A demethylation Clin Cancer Res.
In this article, researchers investigate how neuronal activity and hypoxia impact the progression of glioblastoma, a type of brain cancer. Hypoxia prompts glioma stem cells to activate neurons via glutamate release, furthering cancer development by affecting microglial polarization. The study uncovers the molecular details of this process, involving miR-200c-3p, ZC3H13, and the ERK pathway. Notably, the antiepileptic drug levetiracetam can disrupt this mechanism, potentially offering a novel approach for glioblastoma treatment.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Chen J et. al. (October 2023). ALKBH5 enhances lipid metabolism reprogramming by increasing stability of FABP5 to promote pancreatic neuroendocrine neoplasms progression in an m6A-IGF2BP2-dependent manner J Transl Med. 21(1):741.
The study investigates the role of ALKBH5, an m6A demethylase, in pancreatic neuroendocrine neoplasms (pNENs). ALKBH5 upregulation in pNENs enhances tumor growth and disrupts lipid metabolism. This effect is mediated through the ALKBH5/IGF2BP2/FABP5/mTOR axis, which activates the PI3K/Akt/mTOR signaling pathway. Understanding this mechanism provides insights for potential therapeutic strategies in pNEN treatment.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Li Y et. al. (December 2023). Down-regulation of the FTO gene in follicular fluid of infertile women with ovarian endometriosis Gynecol Endocrinol. 39(1):2269273.
The study examines FTO concentrations in follicular fluid (FF) of women with ovarian endometriosis (OE) undergoing in vitro fertilization-embryo transfer (IVF-ET). Compared to the control group, FTO levels in FF were significantly reduced in the OE group, which could be linked to compromised oocyte quality in endometriosis patients.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Kuschman HP et. al. (October 2023). Nitric oxide inhibits FTO demethylase activity to regulate N6-methyladenosine mRNA methylation Redox Biol. 67:102928.
The article uncovers a novel mechanism by which nitric oxide (NO) inhibits the demethylase activity of FTO, a key enzyme in regulating N6-methyladenosine (m6A) mRNA modifications. This inhibition leads to increased global m6A methylation of mRNA and specific enrichment of m6A in transcripts controlled by NO. The findings highlight a previously unknown role of NO as a modulator of mRNA epigenetic modifications, shedding light on its impact on gene expression and potential implications for various diseases, including cancer.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric), Epigenase m6A Demethylase Activity/Inhibition Assay Kit (Colorimetric)