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EED Monoclonal Antibody [3B12]

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Western Blot detection against Immunogen (37.11 KDa).
Application: ELISA, WB
Areas of Research: Chromatin Remodeling
Clonality: Monoclonal
Conjugate: Unconjugated
Host: Mouse
Isotype: IgG2a Kappa
Purification: Affinity Purified
Reactivity: Human
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-3110-100100 µg $452.00  Discontinued
Availability: Discontinued 
Product Overview

Background
EED (extraembryonic ectoderm) belongs to the Polycomb-group (PcG) family which is known to form multimeric protein complexes involved in preserving the transcriptional repressive state of genes over succeeding cell generations. It can interact with enhancer of zest 2, the cytoplasmic tail of integrin beta7, the HIV-1 MA protein, and histone deacetylase proteins. EED is believed to directly regulate integrin function and can mediate repression of gene activity via histone deacetylation. Multiple variants encoding different isoforms exist for this gene.

Description
Mouse monoclonal antibody raised against a partial recombinant EED, clone 3B12.

Size
100 µg

Purification
Protein A purified

Immunogen
EED (NP_003788, 342 a.a. ~ 442 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG2a Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Alternative Names
Embryonic ectoderm development antibody, Embryonic ectoderm development isoform a antibody, hEED antibody, OTTHUMP00000235483 antibody, OTTHUMP00000235484 antibody, OTTHUMP00000235485 antibody, Polycomb protein eed antibody, WAIT 1 antibody, WD protein associating with integrin cytoplasmic tails 1 antibody

Applications & Suggested Dilutions*
Western Blot: 0.5-1 μg/ml
ELISA: 0.25-1 μg/ml

*The end user is responsible for determining optimal working dilutions.

Note: This item was formerly catalog no. ABB-3013 (ABB3013)

Product Details

Applications

Western Blot

Applicable

Immunoprecipitation Not determined

ELISA

Applicable

Immunohistochemistry (IHC)

Not determined
Immunofluorescence Not determined
User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

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