DTT, also known as Cleland's Reagent, is a particularly strong reducing agent, owing to its high conformational proprensity to form a six member ring with an internal disulfide bond. It has a redox potential of -0.33 V @ pH 7. It is used for many protein research applications, including reducing polyacrylamide electrophoresis (SDS-PAGE). The 500 mM stock solution can be directly used to achieve the amount of reducing agent required for your specific application.
Molecular Weight (kDa)
10 mM phosphate buffered saline, pH 7.4
500 mM, 1 ml
Storage Temp & Conditions
>6 months at 4°C.
Used in biochemistry and various sample preparation applications, works to reduce dissulfide bridges, protects biomolecules, and denatures proteins before electrophoresis analysis (SDS-PAGE). 1-10 mM to maintain reduced proteins in solution, 50-100 mM for complete reduction for electrophoresis.