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CLPTM1L Polyclonal Antibody

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Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: CLPTM1L antibody at 3.7ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 63, 59 KDa
Observed band size: 63 KDa
IHC image of CLPTM1L Polyclonal Antibody diluted at 1:800 and staining in paraffin-embedded human lung cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CLPTM1L Polyclonal Antibody diluted at 1:800 and staining in paraffin-embedded human pancreatic tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with CLPTM1L Polyclonal Antibody at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF, IHC
Areas of Research: Apoptosis & Cell Death
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A70143-02020 µg $99.00 
A70143-05050 µg $249.00 
A70143-100100 µg $399.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
Enhances cisplatin-mediated apoptosis, when overexpressed.

Description
CLPTM1L Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
Q96KA5

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Cleft lip and palate transmembrane protein 1-like protein (32-172AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Cleft lip and palate transmembrane protein 1-like protein, CLPTM1-like protein, Cisplatin resistance-related protein 9, CRR9p, CLPTM1L, CRR9

Application
ELISA, IHC, IF; Recommended dilution: IHC:1:500-1:1000, IF:1:200-1:500

User Guide & MSDS

[User Guide]*
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