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CHRNB2 Polyclonal Antibody

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Western Blot
Positive WB detected in: Jurkat whole cell lysate
All lanes: CHRNB2 antibody at 6.05ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58 KDa
Observed band size: 58 KDa
Immunofluorescence staining of SH-SY5Y cells with CHRNB2 Polyclonal Antibody at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF, IHC
Areas of Research: Signal Transduction
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A68487-02020 µg $99.00 
A68487-05050 µg $249.00 
A68487-100100 µg $399.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodiun ions.

Description
CHRNB2 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
P17787

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Neuronal acetylcholine receptor subunit beta-2 protein (322-458AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Neuronal acetylcholine receptor subunit beta-2, CHRNB2

Application
ELISA, IHC, IF; Recommended dilution: IHC:1:200-1:500, IF:1:50-1:200

User Guide & MSDS

[User Guide]*
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