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CHD4 Polyclonal Antibody

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Western blot analysis of extracts of various cell lines, using CHD4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded Rat liver using CHD4 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human oophoroma using CHD4 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse liver using CHD4 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using CHD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using CHD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U20S cells using CHD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Application: IF, IHC, WB
Areas of Research: Chromatin Remodeling
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-2709-05050 µl $273.00 
A-2709-100100 µl $359.00 
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Product Overview

Background
The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein.

Description
CHD4 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Human

Isotype
IgG

Uniprot ID
Q14839

Purification
Affinity Purified

Immunogen
Recombinant Protein of Human CHD4

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
Mi-2b, Mi2-BETA

Application
WB; Recommended dilution: WB 1:500 - 1:2000

User Guide & MSDS

[User Guide]*
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BisulPlus Loci 5mC & 5hmC Detection PCR kit
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