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CCNY Polyclonal Antibody

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Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: CCNY antibody at 6ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40, 37, 34 KDa
Observed band size: 40 KDa
IHC image of CCNY Polyclonal Antibody diluted at 1:600 and staining in paraffin-embedded human liver tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CCNY Polyclonal Antibody at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF, IHC, WB
Areas of Research: Cell Cycle
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A70111-02020 µg $99.00 
A70111-05050 µg $249.00 
A70111-100100 µg $399.00 
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Product Overview

Background
Positive regulatory subunit of the cyclin-dependent kinases CDK14/PFTK1 and CDK16. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway. Recruits CDK16 to the plasma membrane. Isoform 3 might play a role in the activation of MYC-mediated transcription.

Description
CCNY Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
Q8ND76

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Cyclin-Y protein (2-170AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Cyclin-Y, Cyc-Y, Cyclin box protein 1, Cyclin fold protein 1, cyclin-X, CCNY, C10orf9, CBCP1, CFP1

Application
ELISA, WB, IHC, IF; Recommended dilution: WB: 1:500-1:5000, IHC: 1:500-1:1000, IF: 1:50-1:200

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

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