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CBX4 Polyclonal Antibody

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IHC image of CBX4 Polyclonal Antibody diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CBX4 Polyclonal Antibody diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CBX4 Polyclonal Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Application: ELISA, IF, IHC
Areas of Research: Chromatin Remodeling
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-3016-05050 µg $242.00 
A-3016-100100 µg $399.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
CBX4, or Chromobox protein homolog 4, is part of the polycomb group (PcG) proteins, specifically polycomb repressive complex I. CBX4 acts as a transcriptional suppressor and is a facilitator of SUMO1 conjugation. In addition, it is a candidate of KyoT2-binding proteins and is directly implicated in the cellular mechanisms that occur in response to DNA damage.

Description
CBX4 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid.
0.03% Proclin 300.50% Glycerol, 0.01M PBS, pH 7.4

Specificity
Human

Isotype
IgG

Uniprot ID
O00257

Purification
Protein G purified

Immunogen
Recombinant Human E3 SUMO-protein ligase CBX4 protein (1-160AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
E3 SUMO-protein ligase CBX4 (EC 2.3.2.-) (Chromobox protein homolog 4) (Polycomb 2 homolog) (Pc2) (hPc2), CBX4

Application
ELISA, IHC, IF; Recommended dilution: IHC:1:200-1:500, IF:1:50-1:200

 

VCU0006

User Guide & MSDS

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