DescriptionRat monoclonal antibody to BAFF, Clone BUFFY1
FormulationLiquid. In PBS containing 0.02% sodium azide.
ImmunogenRecombinant human soluble BAFF (aa 83-285)
SpecificityHuman
IsotypeIgG2a
PurificationSerum
StorageStore at +4°C for short term. Aliquot and store at -20°C for long term. Avoid multiple freeze/thaw cycles.
Alternative NamesB cell-activating factor, BLyS, TALL-1, THANK, zTNF4, TNFSF 13B/20, CD257
ApplicationFC, ICC, WB
Fig. 1. Western blot analysis of BAFF Monoclonal Antibody [BUFFY1]: Lane 1: MW marker; Lane 2: BAFF (soluble) (human), (recombinant).
Fig. 2. Flow cytometric profile of 293 cells stably transfected with a BAFF-expression plasmid (1). Control (2) are mock transfected 293 cells. Optimal concentration range is 0.5-5 µg/ml.
Fig. 3. Membrane-bound human BAFF detected by BAFF Monoclonal Antibody [BUFFY1]. Left panel: ICC; Right panel: phase contrast. Lower panel: 293-wt control.