Epigentek Home
 Find Your Distributor      
 

ADAM8 Polyclonal Antibody

Share:

Write a Review
Western Blot
Positive WB detected in: Raji whole cell lysate, THP-1 whole cell lysate, Raw264.7 whole cell lysate
All lanes: ADAM8 antibody at 3.8ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 89, 79, 81 KDa
Observed band size: 89 KDa
IHC image of ADAM8 Polyclonal Antibody diluted at 1:400 and staining in paraffin-embedded human lymph node tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of ADAM8 Polyclonal Antibody diluted at 1:400 and staining in paraffin-embedded human pancreatic tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of MCF-7 cells with ADAM8 Polyclonal Antibody at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF, IHC, WB
Areas of Research: Cell Structure & Function
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human, Mouse
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A69791-02020 µgView
A69791-05050 µgView
A69791-100100 µgView
Product Overview

Background
Possible involvement in extravasation of leukocytes.

Description
ADAM8 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
P78325

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Disintegrin and metalloproteinase domain-containing protein 8 protein (677-824AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Disintegrin and metalloproteinase domain-containing protein 8, ADAM 8, Cell surface antigen MS2, CD156a, ADAM8, MS2

Application
ELISA, WB, IHC, IF; Recommended dilution: WB: 1:500-1:5000, IHC: 1:200-1:500, IF: 1:50-1:200

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

DNMT ELISA Easy Kit
Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2019 Epigentek Group Inc. All rights reserved.

Intro to Epigenetics