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B-actin Monoclonal Antibody [A2-F6]

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Western blot analysis of Beta-actin on different cell lysates using B-actin Monoclonal Antibody [A2-F6] at 1/10000 dilution.
Positive control:
Lane 1: NIH/3T3
Lane 2: PC12
Lane 3: MCF-7
Lane 4: HepG2
Lane 5: Hela
Lane 6: Mouse lung
Immunofluorescent staining of NIH/3T3 cells using B-actin Monoclonal Antibody [A2-F6].
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using B-actin Monoclonal Antibody [A2-F6]. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using B-actin Monoclonal Antibody [A2-F6]. Counter stained with hematoxylin.
Application: FC, ICC, IHC, WB
Areas of Research: Cellular Structure
Clonality: Monoclonal
Conjugate: Unconjugated
Host: Mouse
Isotype: IgG1
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-0800-100100 µl $399.00 
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Product Overview

Background 
Beta actin, or cytoplasmic actin, is a 43kDa highly conserved protein that is ubiquitously expressed in all eukaryotic cells. Actins, along with microtubules, comprise a major component of the cytoskeleton. B-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of Beta-actin is generally used as an internal control for experimental error.

Description
Mouse monoclonal antibody to B-actin, clone A2-F6

Specificity
Human, Mouse, Rat

Isotype
IgG1

Uniprot ID
P60709

Purification
Affinity Purified

Immunogen
Synthetic peptide (KLH-coupled)

Formulation
Liguid. 1*TBS (pH7.4), 0.5% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. 

Storage
Store at 4°C after thawing. Aliquot store at -20°C (regular) or -80°C (long-term). Avoid repeated freeze / thaw cycles.

Alternative Names
Cytoplasmic Actin , ACTB, Actin, Actin, Beta, Cytoplasmic 1, B actin, Beta actin, F actin, β actin, Anti B-actin 

Application
WB: 1:10,000
ICC: 1:100-1:200
IHC: 1:100-1:200
FC: 1:100-1:200

 JHB0002

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