Description
All SUMO isoforms are translated with additional C-terminal residues that have to be removed to generate the active protein. Pro-SUMO-3 (103amino acids) is the inactive precursor of SUMO-3 (92 amino acids) and is processed at the C-terminus by SUMO-3 specific proteases (SENPs). The resulting SUMO-3 protein has the conserved C-terminal Gly-Gly residues that function in activation and conjugation reactions. This protein can be used as a negative control in SUMOylation reactions or as a substrate for SENPs.

Molecular Weight (kDa)
11.6 kDa

Formulation
50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT, 10% glycerol

Source
Expressed in E.coli expression system

Size
100 µg

Storage Temp & Conditions
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.

Purification Notes
Purity: > 90%

Application Notes
Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein; Protein Array; ;Best use within three months from the date of receipt of this protein.