SUMO-3 modified with biotin via primary amine coupling resulting in modification of lysine residues as well as the N-terminus. Although having a fully functional C-terminus, lysine modification may limit the ability of this reagent to propagate poly-SUMO chains. Biotinylated SUMO-3 can be detected using avidin-linked reagents for higher efficiency and sensitivity than with antibodies.
Molecular Weight (kDa)
50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT, 10% glycerol
Expressed in E.coli expression system
Storage Temp & Conditions
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.
Purity: > 95% by SDS-PAGE
Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein; Protein Array; ;Best use within three months from the date of receipt of this protein.