BackgroundVariant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
DescriptionPhospho Histone H2AX (S139) Recombinant Monoclonal Antibody [1F10]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDP16104
PurificationAffinity Chromatography
ImmunogenA synthesized peptide
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesHistone H2AX, H2a/x, Histone H2A.X, H2AFX, H2AX
ApplicationELISA, WB; Recommended dilution: WB:1:3000-1:10000