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PARP1 Monoclonal Antibody [3G4]

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Application: ELISA, IF, WB
Areas of Research: DNA Damage & Repair
Clonality: Monoclonal
Conjugate: Unconjugated
Host: Mouse
Isotype: IgG2a Kappa
Purification: Affinity Purified
Reactivity: Human
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-6130-100100 µg $452.00  Discontinued
Availability: Discontinued 
Product Overview

Description
Mouse monoclonal antibody raised against a partial recombinant PARP1, clone 3G4.

Size
100 µg

Purification
Protein A purified

Immunogen
PARP1 (AAH37545, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG2a Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Alternative Names
ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase), ADP ribosyltransferase diphtheria toxin like 1, ADP ribosyltransferase NAD+, ADPRT, ADPRT1, ARTD1, msPARP, NAD(+) ADP ribosyltransferase 1, pADPRT1, PARP, PARP1_HUMAN, Poly (ADP ribose) polymerase 1, Poly adenosine diphosphate ADP ribose polymerase, Poly ADP ribose polymerase family member 1, Poly ADP ribose synthetase 1, poly(ADP ribosyl)transferase, PPOL

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunofluorescence: 5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (37 KDa).

 

Note: This item was formerly catalog no. ABB-6035 (ABB6035) 

Product Details

Applications

Western Blot


PARP1 Monoclonal Antibody, clone 3G4. Western Blot analysis of PARP1 expression in HeLa NE.

Immunoprecipitation Not determined

ELISA


Detection limit for recombinant GST tagged PARP1 is approximately 0.1 ng/ml as a capture antibody.

Immunohistochemistry (IHC)

Not determined

Immunofluorescence


Immunofluorescence of monoclonal antibody to PARP1 on HeLa cell. [Antibody Concentration: 10 µg/ml]
User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Product Citations

Crawford NP et. al. (October 2009). The metastasis efficiency modifier ribosomal RNA processing 1 homolog B (RRP1B) is a chromatin-associated factor. J Biol Chem. 284(42):28660-73.

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