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Na+/K+-ATPase Monoclonal Antibody new product

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Western blot analysis of lysates from HeLa cells, using Na+/K+-ATPase mAb at 1:50000 dilution. Membrane protein extract isolated from HeLa cells.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of various lysates using Na+/K+-ATPase mAb at 1:50000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using Na+/K+-ATPase mAb at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0. 01M Tris-EDTA Buffer (pH 9. 0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Na+/K+-ATPase mAb at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0. 01M Tris-EDTA Buffer (pH 9. 0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Na+/K+-ATPase mAb at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0. 01M Tris-EDTA Buffer (pH 9. 0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Na+/K+-ATPase mAb at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0. 01M Tris-EDTA Buffer (pH 9. 0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Na+/K+-ATPase mAb at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0. 01M Tris-EDTA Buffer (pH 9. 0) prior to IHC staining.
Confocal imaging of paraffin-embedded Human colon tissue using Na+/K+-ATPase mAb ( dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.
Confocal imaging of MCF7 cells using Na+/K+-ATPase mAb ( dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with a-Tubulin Mouse mAb (dilution 1:400) followed by incubation with conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of paraffin-embedded Mouse kidney tissue using Na+/K+-ATPase mAb ( dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Rat kidney tissue using Na+/K+-ATPase mAb ( dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.
Application: ELISA, IF-P, IF/ICC, IHC-P, WB
Clonality: Monoclonal Antibodies
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A75282-100100 µl $308.00 
Order now & get it by Tuesday, June 9th  
Product Overview

Background
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

Description
Na+/K+-ATPase Monoclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Specificity
Human, Mouse, Rat

Isotype
IgG

Uniprot ID
P05023

Purification
Affinity Purified

Immunogen
Synthetic peptide. Please contact EpigenTek for more information.

Storage
Store at -20°C. Avoid freeze / thaw cycles.

Alternative Names
CMT2DD; HOMGSMR2; Na+/K+-ATPase

Application
WB, IF/ICC, IF-P, IHC-P, ELISA; Recommended dilution: WB, 1:50000 - 1:200000; IF/ICC, 1:100 - 1:1000; IF-P, 1:100 - 1:1000; IHC-P, 1:3000 - 1:12000; ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.

Datasheet & SDS

PDF Datasheet/User Guide: A75282.pdf
If the above file is not available online, you can request a copy by contacting us.
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