MLH1 Monoclonal Antibody

Background
The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC).

Description
MLH1 Monoclonal Antibody. Unconjugated. Raised in: Mouse.

Formulation
Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.

Specificity
Human

Isotype
IgG1, Kappa

Uniprot ID
P40692

Purification
Affinity Purified

Immunogen
Recombinant protein (or fragment). Please contact EpigenTek for more information.

Storage
Store at -20°C. Avoid freeze / thaw cycles.

Alternative Names
FCC2; COCA2; HNPCC; MLH-1; hMLH1; HNPCC2; LYNCH2; MMRCS1; H1

Application
WB, ELISA; Recommended dilution: WB, 1:100 - 1:500; ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.