BackgroundHistone deacetylases (HDACs) play a critical role in transcriptional repression of gene expression in eukaryotic cells through catalyzing the hydrolytic removal of acetyl groups from histone lysine residues. Three distinct families of HDACs have been described, comprising a group of at least 20 proteins in humans. HDAC7 is a class II histone deacetylase.
DescriptionMouse monoclonal antibody raised against a full length recombinant HDAC7A, clone 2B11-1C1.
Size 100 µg
PurificationProtein A purified
ImmunogenHDAC7A (AAH06453, 1 a.a. ~ 277 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross ReactivityHuman
IsotypeIgG2b Kappa
FormulationIn 1x PBS, pH 7.2
StorageAliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.
Handling RecommendationsFor maximum recovery of the products, centrifuge the vial prior to opening the cap.
Alternative NamesDKFZP586J0917 antibody, FLJ99588 antibody, HD7 antibody, HDAC7A antibody, Histone deacetylase 7 antibody, Histone deacetylase 7A antibody, OTTHUMP00000202813 antibody, OTTHUMP00000202814 antibody
Applications & Suggested Dilutions*Western Blot: 0.5-1 µg/mlELISA: 0.25-1 µg/ml
*The end user is responsible for determining optimal working dilutions.
Quality ControlWestern Blot detection against Immunogen (56.47 KDa).
Note: This item was formerly catalog no. ABB-4007 (ABB4007)
Applications
Western Blot
HDAC7A Monoclonal Antibody [2B11-1C1]. Western Blot analysis of HDAC7A expression in K-562.
Western Blot analysis of HDAC7A expression in transfected 293T cell line by HDAC7A monoclonal antibody, clone 2B11-1C1.Lane 1: HDAC7A transfected lysate (29.6 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation
ELISA
Immunohistochemistry (IHC)
Not determined