Goat anti-mouse IgG recognizes mouse IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with biotin.
Liquid; This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.
Mouse IgG whole molecule
In 10 mM sodium phosphate, 75 mM NaCl, 50% (v/v) glycerol, pH 7.2 (0.01% (w/v) sodium azide, 10 mg/mL BSA
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. For maximum recovery of the product, centrifuge the vial prior to opening the cap.
The optimal working dilution should be determined by the end user.
Applications & Suggested Dilutions
Western Blot: 1:1000-1: 10000; Immunohistochemistry:1:100-1:500; Immunofluorescence: 1:100-1:500; ELISA: 1:2000-1:20000.