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FGF21 Monoclonal Antibody new product

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Western blot analysis of various lysates using FGF21 mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of lysates from Mouse liver, using FGF21 mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded Rat lung using FGF21 mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using FGF21 mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using FGF21 mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human kidney using FGF21 mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate buffer (pH 6. 0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using FGF21 mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate buffer (pH 6. 0) prior to IHC staining.
Application: ELISA, IHC-P, WB
Clonality: Monoclonal Antibodies
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A79261-100100 µl $308.00 
Order now & get it by Tuesday, June 9th  
Product Overview

Background
Theis gene encodes a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. This protein is a secreted endocrine factor that functions as a major metabolic regulator. The encoded protein stimulates the uptake of glucose in adipose tissue.

Description
FGF21 Monoclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

Specificity
Human, Mouse, Rat

Isotype
IgG

Uniprot ID
Q9NSA1

Purification
Affinity Purified

Immunogen
Recombinant protein (or fragment). Please contact EpigenTek for more information.

Storage
Store at -20°C. Avoid freeze / thaw cycles.

Alternative Names
FGF21; fibroblast growth factor 21

Application
WB, IHC-P, ELISA; Recommended dilution: WB, 1:500 - 1:2000; IHC-P, 1:50 - 1:200; ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.

Datasheet & SDS

PDF Datasheet/User Guide: A79261.pdf
If the above file is not available online, you can request a copy by contacting us.
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