Epigentek Home
 
EpiGentek Guarantee

EpiQuik Total RNA Isolation Kit

Share:

For isolating total RNA from blood, cultured cells, bacteria, or fungus

Write a Review
Schematic Procedure for the EpiQuik™ Total RNA Isolation Kit.
Total RNA was both isolated from 300 ul bacteria culture.
Input Type: Cells
Research Area: RNA Methylation
Target Application: Sample Isolation
Vessel Format: Columns/Tubes
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-9100-05050 samples $199.00 
Availability: Usually Ships in 1 Day or Same day delivery Same Day NY Delivery 
Product Overview

The EpiQuik™ Total RNA Isolation Kit provides a fast, simple, and cost-effective method for isolating total RNA from whole blood, mammalian cells, bacterial cells, and fungal cells. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows RNA species bases to bind to the glass fiber matrix of the spin column while contaminants pass through the column. Impurities are efficiently washed away, and the pure RNA is eluted with the RE Buffer without phenol extraction or alcohol precipitation. The RNA purified with the Total RNA Isolation Kit is suitable for a variety of routine applications, including the RT-PCR, cDNA Synthesis, Northern Blotting, Differential display, Primer Extension and mRNA selection. The entire procedure can be completed within 25-40 minutes. The kit has the following features and advantages:

  • Fast procedure delivers high-quality total RNA in 25-40 minutes
  • Ready-to-use RNA for high performance in any downstream application
  • Consistent RNA yield from a small amount of starting material

Principle & Procedure
This kit contains all the reagents required for successfully performing RNA isolation directly from blood, cultured cells, or fungus. After lysis, binding, and wash, RNA is easily recovered in quantities of up to 30 µg using specially designed columns. Total RNA is then ready to be used for a variety of downstream applications.

Starting Materials
The amount of starting materials can be up to 300 µl of whole blood, 10^7 mammalian cells, 10^9 bacterial cells, and 10^8 fungal cells. A total of 50 standard extractions can be performed with this kit. Yield of the total RNA can be up to 30 µg. The yield may vary depending on the sample type.

 

Product Components

RL (Pre-lysis Buffer)
RA (Lysis Buffer)
RO (Pre-lysis Buffer-Bacteria/Fungus)
W1 (Wash Buffer-1)
W2 (Wash Buffer-2)*
RE (Elution Buffer)
R-Column
Collection Tubes

*Add 30 ml ethanol (96~100%) to the W2 (Wash Buffer-2), and shake before use.

 

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Epigentek Rewards

Researchers Also Purchased
DNMT1 Monoclonal Antibody [60B1220.1]
DNMT1 Monoclonal Antibody [60B1220.1]
TET3 Polyclonal Antibody
TET3 Polyclonal Antibody
HRP-Goat Anti-Rabbit Secondary Antibody
HRP-Goat Anti-Rabbit Secondary Antibody
TET2 Polyclonal Antibody
TET2 Polyclonal Antibody
Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2017 Epigentek Group Inc. All rights reserved.