The EpiQuik™ Total RNA Isolation Kit provides a fast, simple, and cost-effective method for isolating total RNA from whole blood, mammalian cells, bacterial cells, and fungal cells. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows RNA species bases to bind to the glass fiber matrix of the spin column while contaminants pass through the column. Impurities are efficiently washed away, and the pure RNA is eluted with the RE Buffer without phenol extraction or alcohol precipitation. The RNA purified with the Total RNA Isolation Kit is suitable for a variety of routine applications, including the RT-PCR, cDNA Synthesis, Northern Blotting, Differential display, Primer Extension and mRNA selection. The entire procedure can be completed within 25-40 minutes. The kit has the following features and advantages:
- Fast procedure delivers high-quality total RNA in 25-40 minutes
- Ready-to-use RNA for high performance in any downstream application
- Consistent RNA yield from a small amount of starting material
Principle & Procedure
This kit contains all the reagents required for successfully performing RNA isolation directly from blood, cultured cells, or fungus. After lysis, binding, and wash, RNA is easily recovered in quantities of up to 30 µg using specially designed columns. Total RNA is then ready to be used for a variety of downstream applications.
The amount of starting materials can be up to 300 µl of whole blood, 10^7 mammalian cells, 10^9 bacterial cells, and 10^8 fungal cells. A total of 50 standard extractions can be performed with this kit. Yield of the total RNA can be up to 30 µg. The yield may vary depending on the sample type.
RL (Pre-lysis Buffer)
RA (Lysis Buffer)
RO (Pre-lysis Buffer-Bacteria/Fungus)
W1 (Wash Buffer-1)
W2 (Wash Buffer-2)*
RE (Elution Buffer)
*Add 30 ml ethanol (96~100%) to the W2 (Wash Buffer-2), and shake before use.