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DNMT3L Monoclonal Antibody [B0-5]

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ICC staining DNMT3L in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
ICC staining DNMT3L in NCCIT cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
Immunohistochemical analysis of paraffin-embedded mouse colonic tissue using DNMT3L Monoclonal Antibody [B0-5].
Application: ICC, IHC
Areas of Research: DNA Methylation
Clonality: Monoclonal
Conjugate: Unconjugated
Host: Mouse
Isotype: IgG1
Purification: Protein G Purified
Reactivity: Human, Mouse
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-1800-100100 µl $369.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background 
DNA (cytosine-5)-methyltransferase 3-like is an enzyme that in humans is encoded by the DNMT3L gene. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.

Description
Mouse monoclonal antibody to DNM3TL, clone B0-5

Purification
Protein G purified

Specificity
Mouse, Human

Isotype
IgG1

Immunogen
This antibody is produced by immunizing mice with recombinant protein of DNMT3L.

Formulation
Liguid. 1*TBS (pH7.4), 0.5% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage
Store at 4°C after thawing. Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.

Alternative Names
DNA Cytosine 5 Methyltransferase 3 Like Protein antibody, DNA Methyltransferase 3 Like Protein antibody, DNM3L_HUMAN antibody, Dnmt 3L Antibody, Human Cytosine 5 Methyltransferase 3 like protein antibody, MGC1090 antibody, Anti DNMT3L Molyclonal [B0-5], DNMT3L Monoclonal Antibody

Application
ICC: 1:200-1:500, IHC: 1:200 

JHB0002

 
Fig. 1. ICC staining DNMT3L in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

Fig. 2. ICC staining DNMT3L in NCCIT cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

 
Fig. 3. Immunohistochemical analysis of paraffin-embedded mouse colonic tissue using DNMT3L Monoclonal Antibody [B0-5].

User Guide & MSDS

[User Guide]*
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