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CTRL Monoclonal Antibody new product

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Western blot analysis of various lysates using CTRL mAb at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using CTRL mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M Tris/EDTA Buffer (pH 9. 0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat pancreas using CTRL mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M Tris/EDTA Buffer (pH 9. 0) prior to IHC staining.
Confocal imaging of paraffin-embedded Human pancreas tissue using CTRL mAb ( dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
Confocal imaging of paraffin-embedded Mouse pancreas tissue using CTRL mAb ( dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
Application: ELISA, IF-P, IHC-P, WB
Clonality: Monoclonal Antibodies
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A77934-100100 µl $308.00 
Order now & get it by Tuesday, June 9th  
Product Overview

Background
This gene encodes a serine-type endopeptidase with chymotrypsin- and elastase-2-like activities. The gene encoding this zymogen is expressed specifically in the pancreas and likely functions as a digestive enzyme.

Description
CTRL Monoclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.

Specificity
Human, Mouse, Rat

Isotype
IgG

Uniprot ID
P40313

Purification
Affinity Purified

Immunogen
Synthetic peptide. Please contact EpigenTek for more information.

Storage
Store at -20°C. Avoid freeze / thaw cycles.

Alternative Names
CTRL1; CTRL

Application
WB, IF-P, IHC-P, ELISA; Recommended dilution: WB, 1:5000 - 1:8000; IF-P, 1:50 - 1:200; IHC-P, 1:50 - 1:200; ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.

Datasheet & SDS

PDF Datasheet/User Guide: A77934.pdf
If the above file is not available online, you can request a copy by contacting us.
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