BackgroundThis gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.
DescriptionBRAF V600E Monoclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationBuffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
SpecificityHuman
IsotypeIgG
Uniprot IDP15056
PurificationAffinity Purified
ImmunogenSynthetic peptide. Please contact EpigenTek for more information.
StorageStore at -20°C. Avoid freeze / thaw cycles.
Alternative NamesNS7; B-raf; BRAF1; RAFB1; B-RAF1; BRAF-1; BRAF V600E
ApplicationWB, IF/ICC, ELISA; Recommended dilution: WB, 1:1000 - 1:6000; IF, 1:200- 1 : 800; ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
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