T-bet/Tbx21 Monoclonal Antibody

Background
This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.

Description
T-bet/Tbx21 Monoclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.

Specificity
Human

Isotype
IgG

Uniprot ID
Q9UL17

Purification
Affinity Purified

Immunogen
Recombinant protein (or fragment). Please contact EpigenTek for more information.

Storage
Store at -20°C. Avoid freeze / thaw cycles.

Alternative Names
TBX21; T-PET; T-bet; TBET; TBLYM; T-box 21

Application
WB, IHC-P, FC (intra), ELISA; Recommended dilution: WB, 1:1000 - 1:5000; IHC-P, 1:200 - 1:800; FC (intra), 1:500 - 1:1000; ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.