BackgroundN-alpha acetylation is one of the most frequently occurring protein modifications in humans, occurring in approximately 80% of all human proteins. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and it is required for cells to function properly. The ARD1 gene encodes an N-terminal acetyltransferase that operates as the catalytic subunit of the major amino-terminal acetyltransferase A complex. A mutation in this gene has been linked to Ogden syndrome. Multiple transcript variants exists due to alternative splicing.
DescriptionMouse monoclonal antibody raised against a full length recombinant ARD1, clone 4B7-H4.
Size100 µg
PurificationProtein A purified
ImmunogenARD1 (AAH00308, 1 a.a. ~ 236 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross ReactivityHuman
IsotypeIgG1 Kappa
FormulationIn 1x PBS, pH 7.2
StorageAliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.
Handling RecommendationsFor maximum recovery of the products, centrifuge the vial prior to opening the cap.
Alternative NamesAlpha N acetyltransferase 1A antibody, ARD1 homolog N acetyltransferase (S. cerevisiae) antibody, ARD1A antibody, DXS707 antibody, MGC71248 antibody, N alpha acetyltransferase 10 NatA catalytic subunit antibody, N terminal acetyltransferase complex ARD1 subunit homolog A antibody, Naa10 antibody, NatA catalytic subunit antibody, TE2 antibody
Applications & Suggested Dilutions*Western Blot: 0.5-1 µg/mlELISA: 0.25-1 µg/mlImmunofluorescence: 5 µg/ml
*The end user is responsible for determining optimal working dilutions.
Note: This item was formerly catalog no. ABB-4022 (ABB4022)
Applications
Western Blot
ARD1 Monoclonal Antibody [4B7-H4]. Western Blot analysis of ARD1 expression in Hela NE.
ELISA
Immunohistochemistry (IHC)