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AKR1D1 Polyclonal Antibody

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Western blot analysis of extracts of various cell lines, using AKR1D1 Polyclonal Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Exposure time: 5s.
Application: WB
Areas of Research: Lipid Metabolism Antibodies
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
Trial Size Available: A72331-020
100% Guarantee: 6 months
Catalog No.SizePriceQty
A72331-05050 µl $256.00 
A72331-100100 µl $359.00 
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Product Overview

Background
The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet.

Description
AKR1D1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Human, Mouse, Rat

Isotype
IgG

Uniprot ID
P51857

Purification
Affinity Purification

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human AKR1D1 (NP_005980.1).

Storage
Shipped at 4°C. Upon receipt, store at -20°C. Avoid freeze / thaw cycles

Alternative Names
AKR1D1; 3o5bred; CBAS2; SRD5B1; 3-oxo-5-beta-steroid 4-dehydrogenase

Application
WB; Recommended dilution: WB 1:500 - 1:2000

User Guide & MSDS

[User Guide]*
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