Pseudouridine / 5-ribosyluracil Monoclonal Antibody

Background
Pseudouridine was among the first post-transcriptional modifications discovered and is overall one of the most abundant (1) . It is present in a wide range of cellular RNAs and is highly conserved across species. Psi is derived from uridine (U) via base-specific isomerization catalyzed by Psi synthases. The site-specific pseudouridylation goes through either snoRNA-dependent (requires H/ACA RNP) or -independent mechanism (requires pseudouridine synthase (PUS) family enzymes) (2) . It has an extra hydrogen-bond donor at its non-Watson-Crick edge. When incorporated into RNA, Psi can alter RNA secondary structure by increasing base stacking, improving base pairing and rigidifying sugar-phosphate backbone5. The chemical and physical properties of RNA can be altered with the incorporation of Pseudouridine, which could contribute to subsequent cellular functions.

Description
Pseudouridine / 5-ribosyluracil Monoclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.

Specificity
Species independent

Isotype
IgG

Purification
Affinity Purified

Immunogen
Chemical compounds corresponding to Pseudouridine / 5-ribosyluracil.

Storage
Store at -20°C. Avoid freeze / thaw cycles.

Alternative Names
Pseudouridine; Psi

Application
DB, ELISA; Recommended dilution: DB, 1:1000 - 1:10000; ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.