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Methylamp Hot Taq DNA Polymerase

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Amplification of ~500bp and ~600bp target DNA fragments using Methylamp Hot Taq DNA Polymerase.
Reagent Type: PCR Enzyme
100% Guarantee: 6 months
Catalog No.SizePriceQty
R12015-1500 Units $132.00  Discontinued
R12015-21000 Units $266.00  Discontinued
Availability: Discontinued 
Product Overview

Description
Methylamp Hot Taq DNA Polymerase is a chemically modified Methylamp Taq DNA Polymerase. At ambient temperatures it is inactive, having no polymerization activity. Methylamp Hot Taq DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extentension of non-specifically annealed primers and primer-dimers formed at low temperatures during PCR setup. The enzyme has 5’→3’ polymerization dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.

Concentration
5 units/µl (one unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 min at 74°C).

Source
Purified from an E. coli strain carrying an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.

Storage & Dilution Buffer
50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Composition

  • Methylamp Hot Taq DNA Polymerase 
  • 10x Reaction Buffer B1 (Mg2+ , detergent free): Tris-HCl and (NH4)2SO4 
  • 10x Reaction Buffer B2 (Mg2+ free): Tris-HCl, (NH4)2SO4 and detergent 
  • 25 mM MgCl2
  • 10x Enhancer
    Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). Enhancer should be used at a defined working concentration (1x, 2x or 3x solution). 
Enhancer is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.

Applications

  • Hot Start PCR 
  • Primer extension 
  • TA cloning

Storage Conditions
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this reagent. However, routine storage at -20ºC is strongly recommended.

Fig. 1. Amplification of ~500bp and ~600bp target DNA fragments using Methylamp Hot Taq DNA Polymerase.

1-3 – Methylamp Hot Taq DNA Polymerase

M – EpiQuik 1 Kb DNA Ladder

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

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