Histone H3.3 Monoclonal Antibody

Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.

Description
Histone H3.3 Monoclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.

Specificity
Human, Mouse, Rat, Other (Broad Range)

Isotype
IgG

Uniprot ID
P84243

Purification
Affinity Purified

Immunogen
Synthetic peptide. Please contact EpigenTek for more information.

Storage
Store at -20°C. Avoid freeze / thaw cycles.

Alternative Names
H3F3; H3-3B; H3.3A; H3F3A; BRYLIB1; Histone H3.3

Application
WB, IHC-P, ELISA, ChIP, ChIP-seq; Recommended dilution: WB, 1:1000 - 1:6000; IHC-P, 1:100 - 1:1000; ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.; ChIP, 2µg antibody for 5µg-10µg of Chromatin; ChIP-seq, 1:50 - 1:200