The pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots, as well as for immunogen affinity purification of proteins expressed in E. coli.
Rabbit polyclonal antibody to GST, HRP conjugated
This antibody is produced by immunizing rabbits with GST fusion protein expressed by pGEX-vector.
Liguid. 1*TBS (pH7.4), 0.5% BSA, 40% Glycerol.
Store at 4°C after thawing. Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
GST Antibody, Glutathione-S-Transferase, HRP GST antibody, GST HRP Conjugated, Anti GST HRP Conjugate
WB: 1:5,000-1:10,000, ELISA: 1:10,000
Fig. 1. Western blot analysis on GST fusion protein (26kDa) using GST Polyclonal Tag Antibody, HRP Conjugated.