Differences between LINE-1, ELISA, Other Global DNA Methylation Measurement Methods

Since its discovery in 1950, researchers have been investigating 5-methylcytosine (5-mC), the highly-present epigenetic modification known as DNA methylation. Measuring the total amount of 5-mC or 5-hmC (5-hydroxymethylcytosine) provides insight to researchers to gain understanding into the gene expressions that promote or inhibit significant biological processes. These findings have been found to allow researchers to find biomarkers for diseases and other epigenetic changes that are affected by gene expression.

Throughout the time of these discoveries, a variety of global DNA methylation assays have been developed and utilized as new technology and processes are created to measure the quantitation of overall 5-mC content in DNA samples more accurately and faster than past methods. Today, a wide range of different assays exist, each with their own upsides, limitations, throughput capabilities and input recommendations.

Here we will look at the differences between some of the most widely available methods of global DNA methylation including the advantages, disadvantages and capabilities for the following methods: ELISA, LINE-1, HPLC, LC-MS/MS, M.Sssl Acceptance Assay, LUMA, Bisulfite Sequencing of Repetitive Elements, and End-specific PCR.

Global DNA Methylation Method Comparison