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APOBEC1 Polyclonal Antibody

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Immunofluorescence staining of HepG2 cells with APOBEC1 Polyclonal Antibody at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF
Areas of Research: RNA Processing & Modification
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A69395-02020 µg $99.00 
A69395-05050 µg $249.00 
A69395-100100 µg $399.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
Catalytic component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the APOB mRNA. Also involved in CGA (Arg) to UGA (Stop) editing in the NF1 mRNA. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation.

Description
APOBEC1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
P41238

Purification
>95%, Protein G purified

Immunogen
Recombinant Human C->U-editing enzyme APOBEC-1 protein (1-172AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
C->U-editing enzyme APOBEC-1, Apolipoprotein B mRNA-editing enzyme 1, HEPR, mRNA(cytosine(6666)) deaminase 1, APOBEC1

Application
ELISA, IF; Recommended dilution: IF: 1:50-1:200

User Guide & MSDS

[User Guide]*
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