Antibodies

Primary antibodies are reagents that bind directly to a specific target antigen of interest. They are useful for investigating biomarkers for diseases including cancer, Alzheimer’s, and Parkinson’s. Primary antibodies can directly bind to specific antigens such as peptides, proteins, or other molecules. Various modifications such as DNA methylation, histone acetylation, and phosphorylation can also be studied by using primary antibodies.

What are Secondary Antibodies Secondary antibodies are crucial reagents used in many research applications, particularly in the field of immunology. They are typically used in conjunction with primary antibodies, which are specific to the target antigen of interest. The primary antibody binds to the antigen, while the secondary antibody binds to the primary antibody and provides a means to detect and quantify the antigen-antibody complex. Applications of Secondary Antibodies Secondary antibodies are used in a wide range of techniques such as Western blot, ELISA, Immunohistochemistry, Immunofluorescence, Flow Cytometry, etc. They are extremely important in these techniques because they allow the detection of low levels of antigens, and they also increase the specificity/sensitivity of the assay. Conjugation of Secondary Antibodies Secondary antibodies are commonly conjugated to enzymes such as horseradish peroxidase (HRP) or alkaline phosphatase (AP) to facilitate detection, or to fluorescent dyes for use in fluorescence-based techniques. The use of secondary antibodies also allows for multiplexing, where multiple different antigens can be detected in the same sample simultaneously. What makes buying antibodies from EpiGentek different? At EpiGentek, we supply a collection of over 20,000 individual high-quality antibodies for our customers to ensure that our research customers find exactly what they need for their studies. In addition, we offer a variety of other benefits to help our customers in terms of affordability, convenience, and reliability. See why working with EpiGentek for your antibodies is the right choice: No Shipping & Handling Costs: we’ve waived all shipping and handling fees on our antibodies and antibody panel packs when selecting 2-day shipping (trial sizes not applicable). 100% Application Guarantee: your purchased antibodies are covered for 6-months past the date of purchase. Multiple Conjugates: we carry biotin, FITC and HRP conjugates for nearly all targets. Trial Sizes Available: 10 & 20 µl options available for thousands of antibodies in our catalog.

What are Epitope Tags Epitope tags are short peptide sequences that are added to proteins of interest. The addition of these tags allows for the detection and purification of the tagged protein using specific antibodies (anti-tag antibodies). These tags are generally small and do not affect the function of the protein, making them useful tools in many research applications. Uses of Epitope Tags in Cell Biology and Immunology Epitope tags are used in cell biology and immunology. For example, by adding a tag to a protein, it can be tracked in living cells and even in animals. The most common tags used in this context are GFP, RFP and YFP. These tags are fluorescent proteins and allow the visualization of the location and dynamics of the tagged protein in living cells. Uses of Epitope Tags in Molecular Biology and Biochemistry In molecular biology and biochemistry, epitope tags are commonly used to purify and detect specific proteins. For example, the most common tags are His-tag and Flag-tag, which are small peptides added to the N- or C-terminus of the protein. These tags can be used to purify the protein using anti-tag antibodies coupled to beads. What makes buying antibodies from EpiGentek different? At EpiGentek, we supply a collection of over 20,000 individual high-quality antibodies for our customers to ensure that our research customers find exactly what they need for their studies. In addition, we offer a variety of other benefits to help our customers in terms of affordability, convenience, and reliability. See why working with EpiGentek for your antibodies is the right choice: No Shipping & Handling Costs: we’ve waived all shipping and handling fees on our antibodies and antibody panel packs when selecting 2-day shipping (trial sizes not applicable). 100% Application Guarantee: your purchased antibodies are covered for 6-months past the date of purchase. Multiple Conjugates: we carry biotin, FITC and HRP conjugates for nearly all targets. Trial Sizes Available: 10 & 20 µl options available for thousands of antibodies in our catalog.

Collections of high quality antibodies against select modifications.