All SUMO isoforms are translated with additional C-terminal residues that have to be removed to generate the active protein. Pro-SUMO-2 (95 amino acids) is the inactive precursor of SUMO-2 (93amino acids) and is processed at the C-terminus by SUMO-2 specific proteases (SENPs). The resulting SUMO-2 protein has the conserved C-terminal Gly-Gly residues that function in activation and conjugation reactions. This protein can be used as a negative control in sumoylation reactions or as a substrate for SENPs.
Molecular Weight (kDa)
50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT, 10% glycerol
Expressed in E.coli expression system
Storage Temp & Conditions
Store at -80°C. Avoid multiple freeze/ thaw cycles.
Purity: >95% by SDS-PAGE
Typical in vitro concentrations are 10-50 μM depending on conditions