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Methylamp Hot Taq EvaGreen qPCR Mix (no ROX)


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Reagent Type: PCR Mix
100% Guarantee: 6 months
Catalog No.SizePriceQty
R12028-11 ml $143.00  Discontinued
Availability: Discontinued 
Product Overview

Methylamp Hot Taq EvaGreen® qPCR Mix (no ROX) is optimized for real-time quantitative PCR assays. The ready-to-use mix includes Methylamp Hot Taq DNA polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. Only water, template and primers need to be added. Methylamp Hot Taq DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

EvaGreen® Dye: DNA-binding dye for qPCR that, compared to SYBR® Green I, has similar spectra but much less PCR inhibition. It is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!

Methylamp Hot Taq EvaGreen® qPCR Mix (no ROX) can be used with all major qPCR cyclers that do not require reference dye, including Bio-Rad iQ™ 5, Opticon™, Opticon™ 2; Chromo 4™, MiniOpticon, CFX96, CFX384; Corbett Rotor-Gene™ 3000, Rotor-Gene™ 6000; Roche LightCycler® 480 and Techne Quantica™.


  • Methylamp Hot Taq DNA polymerase
  • 5 x qPCR Buffer E
  • 12.5 mM MgCl2: 1 x PCR solution – 2.5 mM MgCl2
  • dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP
  • EvaGreen® dye
  • no ROX dye
  • 1 ml of mix is sufficient for 250 reactions


  • Detection and quantification of DNA and cDNA targets including DNA from bisulfite treatment, ChIP or MeDIP
  • Profiling gene expression 
  • High Resolution Melt (HRM) Analysis 
  • Microbial detection 
  • Viral load determination

Storage Conditions
Routine storage: -20°C. Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of Methylamp Hot Taq EvaGreen® qPCR Mix (no ROX).

User Guide & MSDS

[User Guide]*
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